How can we extract DNA?

In MTC we did an experiment to understand how we can extract DNA from natural products and to observe the fibrilar structure of DNA.

We mixed pieces of the central part of an onion with 3 tsp of detergent, 1 tsp of salt and distilled water and beat the solution.

1

We then filtrated the solution with a coffee filter.

2

We filled 1/4 of a test tube with the filtrate and mixed it thoroughly with some pieces of banana to which we then added the same volume of ethanol.

3

We let it rest for 2-3 minutes until a cloudy area is formed between the 2 layers.

4 5

Finally, we extracted a network of white fibres of DNA and place it on a Petri dish and added a few drops of blue methylene.

6 7 8