Enzymes and Temperature

In our biology class, we started studying enzymes. In order to see how the Catalase works, our teacher told us to do an experiment.

AIM: how temperature affects the activity of catalase.

This was the task:

1- Write down the equation of the chemical reaction in words and build a table to record your results.

2- The teacher provides you with:

a- 3 test tubes filled with hydrogen peroxide (substrate)

b- a piece of potato (source of catalase)

3- Cut the potato into 2 cubes of 0.5 cubic centimeters each. The third cube has already been prepared for you.

4- Place one cube and one test tube in the 1st water bath at 5°C for 2 minutes.

5- Place the cube into the test tube and leave it in the water bath for 2 minutes.

6- Measure the height of the foam produced.

7- Record the result in the table.

8- Repeat steps 4 to 7 with the other cubes.

9- Plot the information in a line-graph


a- Why did you leave the potato and the test tube in the water baths before the experiment?

b- State: – the independent variable

The temperature.

– the dependent variable

The height of the foam produced.

– two fixed variables

c- Describe the results shown in the graph.

d- At what temperature the enzyme works best?

At 30ºC.

e- What has happened at 100°C?

No foam is produced. There is no activity because at high temperatures the active site loses its shape, therefore the substrate doesn’t fit into the enzyme. The enzyme is denatured.

Fiscal sense of balance

Fiscal sense of balance

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